The production of medical test paper kits tells you the types of kits available

The production of medical test paper kits tells you that, first, according to the detection principle, the main reagents are biochemical reagents, immune reagents and molecular reagents. The principle of biochemical reagents is generally to react with reagent components according to the biochemical properties of the tested substance (also similar to the biochemical reaction of enzyme-catalyzed chemical reaction; however, biochemical reagents also include immunoturbidimetric reagents based on antigen-antibody reaction), and then select indicator reagents according to the results of the biochemical reaction to indicate the content of the substance to be tested. The principle of immune reagent is to make the tested substance (usually antigen or antibody) react with the reagent component through the antigen-antibody reaction, and then determine the content by indicating the reagent indicator.

The production of medical test paper kit tells you that the principle of molecular reagent is to make the substance to be tested (generally amplified single-stranded DNA or RNA) react with the reagent component through the principle of base complementary pairing, and then judge the content by indicating reagent. The characteristic of these reagents is that they correspond to different properties of the substance to be tested and can be designed from different methodological perspectives. Sometimes, the same substance to be tested can also be tested with different methods of reagents. These technologies come from laboratory analysis technologies, such as the recently emerging mass spectrometry detection technology (and related chromatography-mass spectrometry tandem detection technology), which has been separated from the category of "diagnostic reagents" and is a new detection technology. According to different indicator reaction principles and reagent forms, there are also other classification methods. For example, among the immune reagents, enzyme-linked immunosorbent assay (ELISA), chemiluminescence reagent (CLIA), fluorescent immunoassay (FIA) and colloidal gold (gold) reagents are currently popular in the market. These reagents are mainly different in indicator reagents, but the reaction with the substance to be tested belongs to immune reaction, so they all belong to immune reagents. The characteristic of these reagents is that although the substances and reagent components to be tested are combined by immune reaction, different instruments will be used for analysis according to different signals due to different indicator reagents. For example, the colloidal gold reagent is used as a signal by directly observing the gold particle sol bound to the protein. This signal is visible to the naked eye and does not need interpretation equipment qualitatively, so it is usually used for rapid diagnosis, such as early pregnancy reagent and COVID-19 reagent this year.

The production of medical test paper kit tells you that the reagent usually uses horseradish peroxidase (HRP) for color reaction (the specific principle can be Baidu), and requires the microplate reader to detect the absorbance of the corresponding wavelength, which is used to indicate the content of the substance to be tested. These indicator reactions will affect the performance of the reagent to a certain extent. Therefore, some different types of reagents are suitable for rapid detection, some for quantitative detection, some for high-throughput screening, and some for bedside diagnosis. According to different product requirements, different reagents will use different methods.